논문
2024
Choi SJ, Park YS, Lee KW, Park YJ, Jang HJ, Kim DM, Yoo TH
"Sensitive methods to detect single-stranded nucleic acids from food pathogens based on cell-free protein synthesis and retroreflection signal detection" J. Agric. Food Chem. (Accepted)
Lee SJ, Kim DM
"Cell-Free Synthetic Biology: Navigating the New Frontiers of Biomanufacturing and Biological Engineering" Curr. Opin. Syst. Biol. 37: 100488
Yeom KH, Park YJ, Kim HS, Song DY, Kim DM, Park JH
"Photothermal heating of cell-free reactions for on-site production of recombinant proteins" Biotechnol. Bioprocess Eng. (Accepted)
Kim KJ, Lee SJ, Kim DM
"Use of antioxidants to extend the storage of lyophilized cell-free synthesis system" Biotechnol. Bioprocess Eng. (Accepted)
2023
Lee JH, Song DY, Lim HJ, Kim DM
"A cell-free protein synthesis method for the detection of heavy metal ions using a personal glucose meter" Biotechnol. Bioprocess Eng. 28: 137-142
Kim KJ, Lee SJ, Kim DM
"The Use of Cell-free Protein Synthesis to Push the Boundaries of Synthetic Biology" Biotechnol. Bioprocess Eng. 28: 922-928
Han J, Lim HJ, Park J, Han DH, Kim DM, Park JK
"On-chip microfluidic dual detection of amino acid metabolism disorders using cell-free protein synthesis" Biosens. Bioelection. 222: 114936
Song DY, Park YJ, Kim DM
"A one-pot transcriptional assay method that detects the tumor biomarker FEN1 based on its flap cleavage activity" Anal. Chim. Acta 1282: 341928
Lee DG, Lim HJ, Lee HY, Kim DM, Lee KH, Byun JY, Shin YB
"A controlled transcription-driven light-up aptamer amplification for nucleotide triphosphate detection" Biochip J. 17: 487-495
Park YJ, Song DY, Kim DM
"TASER: A flexible approach for nucleic acid detection using a molecular converter" Sens. Actuators B Chem. 400, Part B: 134932
Yoo DB, Hong SJ, Yun SH, Kang MJ, Cho BK, Lee HK, Choi HK, Kim DM, Lee CG
"Metabolic Engineering for Redirecting Carbon to Enhance the Fatty Acid Content of Synechocystis sp. PCC6803" Biotechnol. Bioprocess Eng. 28: 274-280
2022
Lee YJ, Lim HJ, Kim DM
"Quantitative analysis of -Aminobutyric acid by combined cell-free protein synthesis and transamination reactions" ACS Synth. Biol. 11: 1208-1212
Oh JY, Oh YR, Kim DM, Eom GT, Song JK
"Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks" Curr. Opin. Biotechnol. 73: 158-163
Lee HY, Kim DM
"Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks" Curr. Opin. Biotechnol. 73: 158-163
Lee YJ, Lee SJ, Kim DM
"Translational detection of indole by complementary cell-free protein synthesis assay" Front. Biotechnol. Bioeng 10: 900162
Hong YS, Kim DE, Park YJ, Kim DM, Byun JY, Shin YB
"MicroRNA detection using light-up aptamer amplification based on nuclease protection transcription" ChemComm 58: 2359-2362
2021
Kang MJ, Hong SJ, Yoo D, Cho BK, Lee H, Choi HK, Kim DM, Lee CG
"Photosynthetic production of biodiesel in Synechocystic sp. PCC6803 transformed with insect of plant fatty acid methyltransferase" Biotechnol. Bioprocess Eng. 44: 1433-1439
Lee HH, Noh YJ, Hong SJ, Lee H, Kim DM, Cho BK, Lee CG, Choi HK
"Photosynthetic pigment production and metabolic and lipidomic alterations in the marine cyanobacteria Synechocystis sp. PCC 7338 under various salinity conditions" J. Appl Phycol 33: 197-209
Park YJ, Kim DM
“Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System" Front. Bioeng. Biotechnol. 9: 778496
Jeong Y, Hong SJ, Cho SH, Yoon S, Lee H, Choi HK, Kim DM, Lee CG, Cho S, Cho BK
"Multi-omic analyses reveal habitat adaptation of marin cyanobacterium Synechocystis sp.PCC 7338" Front. Microbiol. 12: 667450
Noh YJ, Lee H, Kim M, Hong SJ, Lee H, Kim DM, Cho BK, Lee CG, Choi HK
"Enhanced production of photosynthetic pigments and various matebolites and lipids in the cyanobacteria Synechocystis sp. PCC 7338 culture in the presence of exogenous glucose" Biomolecules 11(2): 214
Jang SA, Park JH, Lim HJ, Oh JY, Bae KH, Lee KJ, Song JK, Kim DM
"Bio-specific immobilization of enzymes on electrospun PHB nanofibers" Enzyme Microb. Technol. 145: 109749
Sim J, Baek MS, Lee KH, Kim DM, Byun JY, Shin YB
"A highly sensitive and versatile transcription immunoassay using a DNA-encoding tandem repetitive light-up aptamer" Talanta 224: 121921
Baek MS, Lee KH, Byun JY, Shin YB, Kim DM
"Aptamer-linked in vitro expression assay for ultrasensitive detection of biomarkers" Anal. Chem. Acta 1146: 118-123
2020
Lee H, Noh YJ, Hong SJ, Lee H, Kim DM, Cho BK, Lee CG, Choi HK
"Photosynthetic pigment production and metabolic and lipidomic alterations in the marine cyanobacteria Synechocystis sp. PCC 7338 under various salinity conditions" J. Appl. Phycol.
Jeong Y, Cho SH, Lee H, Choi HK, Kim DM, Lee CG, Cho S, Cho BK
"Current status and future strategies to increase secondary metabolite production from cyanobacteria" Microorganisms 8: 1849
Kwon D, Park JM, Duong VA, Hong SJ, Cho BK, Lee CG, Choi HK, Kim DM, Lee H
"Comparative proteomic profiling of marine and freshwater synechocystis strains using liquid chromatography-tandem mass spectrometry" J. Mar. Sci. Eng. 8: 790
Lim HJ, Jang YJ, Lee KH, Kim DM
"Translational detection of nonproteinogenic amino acids using an engineered complementary cell-free protein synthesis assay" Anal. Chem. 92(17): 11505-11510
Lee HW, Baek JI, Kim SJ, Kwon KK, Rha E, Yeon SJ, Kim HS, Lee DH, Kim DM, Lee SG
"Sensitive and rapid phenotyping of microbes with soluble methane monooxygenase using a droplet-based assay" Front.Bioeng. Biotechnol. 8: 358
2019
Lee KH, Kim DM
"In vitro use of cellular synthetic machinery for biosensing applications" Front. Pharmacol. 10: 1166
Lim HJ, Kim DM
"Cell-free metabolic engineering: Recent developments and future prospect" Methods Protoc. 2(2):
Yang HJ, Lee KH, Lim HJ, Kim DM
"Tandem cell-free protein synthesis as a tool for rapid screening of optimal molecular chaperones" Biotechnol. J. 14: 1800523
이소정, 김호철, 김동명
"지질의 첨가를 통한 포도당 기반 무세포 단백질 합성 시스템의 단백질 발현 효율 향상" Korean Chem. Eng.
Res. 57(1): 85-89
Jang YJ, Lee KH, Yoo TH, Kim DM
"Interfacing a personal glucose meter with
cell-free protein synthesis for rapid analysis of amino acids" Anal. Chem.
91(3): 2531-2535
Byun JY, Lee KH, Shin YB, Kim DM
"Cascading amplification of immunoassay signal by cell-free expression of firefly luciferase from detection antibody-conjugated DNA in an Escherichia coli extract" ACS Sens. 4(1): 93-99
Moon BJ, Lee KH, Kim DM
"Effect of ATP regeneration systems on the yields and solubilities of cell-free synthesized proteins" J. Ind. Eng. Chem. 70: 276-280
2018
Go SY, Lee KH, Kim DM
"Detergent-assisted enhancement of the translation rate during cell-free synthesis of peptides in an Escherichia coli extract" Biotechnol. Bioprocess Eng. 23(6): 679-685
Yi T, Lim HJ, Lee SJ, Lee KH, Kim DM
"Synthesis of (R,R)-2,3-butanediol from starch
in a hybrid cell-free reaction system" J. Ind. Eng. Chem. 67: 231-235
Yeom SJ, Kim MJ, Kwon KK, Fu Y, Rah E, Park SH, Lee HW, Kim HS, Lee DH, Kim DM, Lee SG
"A synthetic microbial biosensor for
high-throughput screening of lactam biocatalysts" Nat. Commun. 9: 5053
Lim HJ, Lee KH, Kim DM
"Rapid determination of effective folding
agents by sequential cell-free protein synthesis" Biochem. Eng. J. 138:
106-110
Lee KH, Kim DM
"Recent advances in development of cell-free
protein synthesis systems for fast and efficient production of recombinant
proteins" FEMS Microbiol. Lett. 365(17)
Yeom SJ, Kim MJ, Kim SK, Lee DH, Kwon KK, Lee HW, Kim HS, Kim DM, Lee SG
"Molecular and biochemical characterization of
a novel isoprene synthase from Metrosideros polymorpha" BMC Plant Biol. 18(1): 118
Zahra Z, Kim SY, Kim HY, Lee H, Lee H, Jeon JY, Kim DM, Kim DM, Hong SJ, Cho BK, Lee H, Lee CG, Arshad M, Choi HK
"Phycobiliproteins production enhancement and lipidomic alteration by titanium dioxide nanoparticles in Synechocystis sp. PCC 6803 culture" J. Agric. Food Chem. 66(32): 8522-8529
2017
Park YJ, Lee KH, Baek MS, Kim DM
"High-throughput engineering of initial coding
regions for optimal production of recombinant proteins" Biotechnol.
Bioprocess Eng. 22(5): 497-503
Jang YJ, Lee KH, Yoo TH, Kim DM
"Complementary cell-free translational assay
for quantification of amino acids" Anal. Chem. 89: 9638-9642
Kasi D, Catherine C, Lee SW, Lee KH, Kim DM
"Cell-free translational screening of an
expression sequence tag library of Clonorchis sinensis for novel antigen
discovery" Biotechnol. Progress 33(3): 832-837
Kasi D, Na HJ, Catherine C, Kim ES, Han KB, Ha JC, Kim DM
"Enhanced production of soluble recombinant
proteins with an in situ-removable fusion partners in a cell-free synthesis
system" Biotechnol. J. 12: 1700125
Park YJ, Lee KH, Kim DM
"Assessing translational efficiency by a
reporter protein co-expressed in a cell-free synthesis system" Anal.
Biochem. 518: 139-142
Lee KH, Kwon JH, Kim DM
"Direct translational analysis of electrophoretically separated DNA using a cell-free protein synthesis system" J. Ind. Eng. Chem. 45: 237-240
2016
Praveenkumar R, Kim BH, Lee JY, Vijayan D, Lee KB, Nam BR, Jeon SG, Kim DM, Oh YK
"Mild pressure induces rapid accumulation of
neutral lipid (triacylglycerol) in Chlorella spp." Bioresour. Technol.
220: 661-665
Kim BH, Praveenkumar R, Lee JY, Nam BR, Kim DM, Lee KB, Lee YC, Oh YK
"Magnesium aminoclay enhances lipid production
of mixotrophic Chlorella sp. KR-1 while reducing bacterial populations"
Bioresour. Technol. 219: 608-613
Bae JS, Jeon EK, Moon SY, Oh WS, Han SY, Lee JH, Yang SY, Park JW, Kim DM
"Bicontinuious nanoporous frameworks: Caged
longevity for enzymes" Angew. Chem. 128(38): 11667-11670
Kim EJ, Song MC, Kim MS, Beom JY, Lee EY, Kim DM, Nam SJ, Yoon YJ
"Characterization of the two methylation step
involved in the biosynthesis of mycinose in tylosin" J. Nat. Prod. 79(8):
2014-2021
Lee KB, Hou CY, Kim CE, Kim DM, Suga H, Kang TJ
"Genetic code expansion by degeneracy reprogramming of arginyl codons" Chembiochem 17(13): 1198-1201
Lim HJ, Park YJ, Jang YJ, Choi JE, Park JH, Song JK, Kim DM
"Cell-free synthesis of functional
phospholipase A1 from Serratia sp." Biotechnol. Biofuels 9: 159
Lee KH, Chatherine C, Kim DM
"Enhanced production of unnatural amino acid-containing proteins in a cell-free protein synthesis system" J. Ind. Eng. Chem. 37: 90-94
Min SE, Lee KH, Park SW, Yoo TH, Oh CH, Park JH, Yang SY, Kim YS, Kim DM
"Cell-free production and streamlined assay of cytosol-penetrating antibodies" Biotechnol. Bioeng. 113(10): 2107–2012
Lee KH, Min SE, Kim HS, Lee SG, Kim DM
"A molecular nanodevice for targeted degradation of mRNA during protein
synthesis" Sci. Rep. 6: 20733
2015
Kim JS, Choi DK. Park SW, Shin SM, BAE JM, Yoo TH, Kim YS, Kim DM
"Quantitative assessment of cellular uptake
and cytosolic access of antibody in living cells by an enhanced split GFP
complementation assay" Biochemical Biophysical Research Communications
467(4) : 771-777
Catherine C, Lee SW, Ju JW, Kim HC, Shin HI, Kim YJ, Kim DM
"Cell-free expression and in situ immobilization of parasite proteins from Clonorchis sinensis for rapid identification of antigenic candidates" PLoS One 10(11): e0143597
Kim HC, Kim KS, Kang TJ, Choi JH, Song JJ, Choi YH, Kim BG, Kim DM
"Implementing bacterial acid resistance into
cell-free protein synthesis for buffer-free expression and screening of
enzymes" Biotechnol. Bioeng. 112(12): 2630-2635
Catherine C, Oh SJ, Lee KH, Min SE, Won JI, Yun H, Kim DM
"Engineering thermal properties of
elastin-like polypeptides by incorporation of unnatural amino acids in a
cell-free protein synthesis system" Biotechnol. Bioprocess Eng. 20:
417-422
2014
Yoon HD, Catherine C, Hong SJ, Kim HC, Lee KH, Oh SJ, Kim DM
"Translational incorporation of multiple amino acids in a cell-free protein synthesis system" Biotechnol. Bioprocess Eng. 19(3): 426-432
Park JY,Nah JG, Oh YK, Jeon SG, Seo JY, Kim BH, praveenkumar R, Lee SY, Lee KB, Kim DM
"Repeated use of stable magnetic flocculant
for efficient harvest of oleaginous Chlorella sp." Bioresour Technol 167:
284-290
Park SH, Ko YJ, Ashok S, Zhou S, Kim DM
"Development of a deletion mutant of
Pseudomonas denitrificans that does not degrade 3-hydroxypropionic acid"
Appl. Microbiol. Biotechnol. 98(10): 4389-4398
Kwon YC, Song JK, Kim DM
"Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems" Method Mol. Cell. Biol. 97-108
2013
Oh YK, Jang WS, Praveenkumar R, Jeon SG, Nah JG, Lee SY, Lee KB, Kim DM
"Magnetophoretic harvesting of oleainous
Chlorella sp. by using biocompatible chitosan/magnetic nanoparticle
Lee EY, Sim JW, Lee KH, Ahn JH, Kim DM
"Streamlined cell-free protein synthesis from
sequence information"Biotechnol. Bioprocess Eng. 18(6): 1101-1108
Oh SJ, Lee KH, Catherine C, Kim DM
"Cell-free platforms for flexible expression
and screening of enzymes" Biotechnol Adv 31(6): 797-803
Lee KH, Kim DM
"Applications of cell-free protein synthesis
in synthetic biology" Biotechnol J 8(11): 1246-1363
Kim MG, Choi DH, Park JH, Li T, Shin YB, Byun JY, Kim DM
"The use of an engineered single chain
variable fragment in a localized surface plasmon resonance method for analysis
of the C-reactive protein" Chem Commun 49(82): 9497-9499
Kim BK, Lee EY, Yoon YJ, Oh IS, Lee KH, Lee NH, Kwon YC, Kim DM
"Integrating cell-free biosyntheses of heme
prosthetic group and apoenzyme for the synthesis of functional P450
monooxygenase" Biotechnol. Bioeng. 110(4): 1193-1200
Lee KY, Kim MG, Lee KH, Byun JY, Kim DM
"In-gel expression and in situ immobilization
of proteins for generation of three dimensional protein arrays in a hydrogel
matrix" Lab Chip 13(5): 886-891
Lee TS, Lee KW, Seo BK, Kim DW, Jang KS, Kim DK, Seo SW, Kim DM
"Fluorescence energy transfer between
polydiacetylene vesicles and embedded benzoxazole molecules for pH
sensing" React Funct Polym 73(3): 451-456
Kim MG, Shin YB, Byun JY, Kim DM
"A colorimetric homogeneous immunoassay system
for the C-reactive protein" Analyst 138(5): 1538-1543
Lee SH, Kwon YC, Park CB, Kim DM
"Cytochrome P450-catalyzed o-dealkylation coupled with photochemical NADPH regeneration" Biotechnol. Bioeng. 110(2): 383-390
2012
Kang TJ, Kim HC, Suga H, Lee KB, Kim DM
"Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming"J Biotechnol 164(2): 330-335
Lee EY, Yoon YJ, Kim HS, Choi SH, Kim DM
"Glycosyltransferase and its application to glycodiversification of natural products"Ind Eng Chem 18(4): 1208-1212
Kim BK, Byun JY, Lee KY, Lee KH, Kim DM
"On-bead expression of recombinant proteins in an agarose gel matrix coated on a glass slide" Lab Chip 12(9): 1605-1610
Park JU, Lee KH, Lee KY, Kim DM
"Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids" PLoS One 7(3): e34429-
Lee EY, Yoon YJ, Mili R, Choi SH, Kim DM
"Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes" Biotechnol Lett 34(3): 499-505
Lee KH, Kin KE, Kim DP, Asthana A, Kim DM
"Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using "modified" embedded template method" Biomicrofluidics 6(1): 012821-
2011
Kim DM, Lee KH, Kwon YC, Kim HC
"Multi-hour translation of mRNA in a cell-free
system" Biotechnol. Bioprocess Eng. 16(6): 1152-1156
Kim DM, Keum JW, Ahn JH
"Expression Screening of Fusion Partners from
an E. coli Genome for Soluble Expression of Recombinant Proteins in a Cell-Free
Protein Synthesis System" PLoS One 6(11)
Kim DM, Kim TW, Kim HC
"Prolonged production of proteins in a
cell-free protein synthesis system using polymeric carbohydrates as an energy
source" Process Biochem 46(6): 1366-1369
Yoon YJ, Kim BG, Kim DM, Lee EY, Park JW, Park SR, Han AR
"Biosynthesis of Glycosylated Derivatives of Tylosin in Streptomyces venezuelae" J. Microbiol. Biotechnol. 21(6): 613-616
Won JI, Kim BK, Park SY, Yoem J, Choo HS, Kim DM
"A new cloning strategy for generating
multiple repeats of a repetitive polypeptide based on non-template PCR"
Biotechnol Lett 33(5): 977-983
Song JK, Kwon MA, Park CG, Kim DM
"Cell-Free Synthesis and Multifold Screening of Candida Antarctica Lipase B (CalB) Variants After Combinatorial Mutagenesis of Hot Spots" Biotechnol. Prog. 27(1): 47-53
2010
Won JI, Kim BK, Park JE, Chu HS, Kim DM
"The effects of supplementing specific amino
acids on the expression of elastin-like polypeptides (ELPs)" Protein Expr.
Purif. 74(2): 298-303
Kim BG, Seo JH, Han KB, Kim HC, Lee KH, Kwon YC, Kim DM
"Cloning-independent expression screening of w-transaminases using a cell-free protein synthesis system" Appl. Environ. Microbiol. 76(18): 6295-6298
Lee TS, Lee MJ, Lee CS, Kwak CK, Kim DM
"Aldehyde-functinalized, water-soluble
poly(para-phenylene): Synthesis and streptavidin assay using FRET"J
Nanosci Nanotechnol 10(10): 6920-6924
Yoo SJ, Kwon YC, Lee KH, Kim DM
"Ribosomal synthesis and in situ isolation of
peptide molecules in a cell-free translation system" Protein Expr. Purif.
71(1): 16-20
Lee JC, Chung JH, Lee MS, Oh IS, Kim DM
"Cell-free production of functional antibody
fragments" Bioprocess Biosyst Eng 33: 127-132
Byun JY, Kim DM
"A simple and cost effective method to prepare
template DNAs for cell-free protein synthesis"Korean J Chem Eng 27:
179-182
Won JI, Kim BG, Park JE, Lee KH, Chu HS, Kim DM
"Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system" Enzyme Microb. Techonol. 46: 87-91
2009
Kim HC, Kim DM
"Methods for energizing cell-free protein
synthesis"J. Biosci. Bioeng. 108(7): 1-4
Pack CB, Kim KE, Lim SE, Kim DM
"Silica-coated alginate beads for in vitro
protein synthesis via transcription/translation machinery encapsulation" J
Biotechnol 143(3): 183-189
Kim DM
"무세포 단백질
합성 기술의 연구동향" News and Information for Chemical Engineeres
27: 650-656
Ki DM, Song JK, Oh IS, Kim TW, Park CG
"Expression of Functional Candida antarctica
Lipase B in a Cell-Free Protein Synthesis System Derived from Escherichia
coli" Biotechnol. Prog. 25: 589-593
Kim DP, Kim DM, Perumal J, Kim KO, Asthana A
"Facile single step fabrication of
microchannels with varying size" Lab Chip 9: 1138-1142
Kim DM, Park CB, Lee SH, Hahn GH, Lim SY, Kim KO
"Cell-Free Synthesis of Functional Proteins
Using Transcription/Translation Machinery Entrapped in Silica Sol-Gel
Matrix" Biotechnol. Bioeng. 102: 303-307
Kim DM, Kang TJ, Ahn JH, Keum JW
"Combinatorial, Selective and Reversible
Control of Gene Expression Using Oligodeoxynucleotides in a Cell-Free Protein
Synthesis System" Biotechnol. Bioeng. 102: 577-582
2008
Kim DM, Oh IS, Kim HC, Kim TW
"A highly efficient and economical cell-free
protein synthesis system using the S12 extract of Escherichia coli"
Biotechnol. Bioprocess Eng. 13: 464-469
Kim DM, Park K, Oh IS, Park CG, Kim TW, Kim HC
"Continuous cell-free protein synthesis using
glycolytic intermediates as energy sources" J Microbiol. Biotechnol. 18:
885-888
Kim DM, Keum JW, Ahn JH
"High-throughput, combinatorial engineering of
initial codons for tunable expression of recombinant proteins" J. Proteome
Res. 7: 2107-2113
Kim DM, Kang TJ, Ahn JH
"Tuning the expression level of recombinant
proteins by modulating mRNA stability in a cell-free protein synthesis
system" Biotechnol. Bioeng. 101: 422-427
Kim DM, Huh KM, Hahn GH, Kwon YC
"Synthesis of functional proteins using
Escherichia coli extract entrapped in calcium alginate microbeads"Anal.
Biochem. 373: 192-196
Kim DM, Byun JY
"At in vitro strategy for the rapid expression of recombinant proteins at low temperature" Biotechnol. Bioprocess Eng. 13(1): 84-88
2007
Kim DM
"Use of L-Buthionine Sulfoximine for the
efficient expression of disulfide-containing proteins in cell-free extracts of
Escherichia coli" Biotechnol. Bioprocess Eng. E 12(0): 574-578
Kim DM, Byun JY, Kim KO
"Preparation of template plasmids for
cell-free protein synthesis using a regenerated anion-exchange column"
Biotechnol. Bioprocess Eng. 12: 562-565
Kim DM, Choi CY, Lee KH, Byun JY, Kwon YC, Keum JW, Oh IS, Kim TW
"Prolonged cell-free protein synthesis using
dual energy sources: Combined use of creatine phosphate and glucose for the
efficient supply of ATP and retarded accumulation of phosphate"
Biotechnol. Bioeng. 97: 1510-1515
Kim DM, Kim HC, Choi CY, Oh IS, Keum JW, Kim TW
"An economical and highly productive cell-free
protein synthesis system utilizing fructose-1,6-bisphosphate as an energy
source" J Biotechnol 130: 389-393
Kim DM, Kim MG, Shin YB, Oh IS, Kim KO, Ahn JH, Joung HA, Kee KH
"Real-time monitoring of cell-free protein
synthesis on a surface plasmon resonance chip"Anal. Biochem. 366: 170-174
Kim DP, Kim DM, Asthana A, Hahn GH
"A continuous-exchange cell-free protein
synthesis system fabricated on a chip" Anal. Biochem. 365: 280-282
Jung GY, Kim DM, Kim TW, Ahn JH, Chu HS, Hwang S, Seo SW, Park YS
"Design of 5 '-untranslated region variants
for tunable expression in Escherichia coli" Biochem Biophys Res Commun.
356: 136-141
Kim DM, Choi CY, Lee KH, Hwang MY, Ahn JH
"Use of signal sequences as an in situ
removable sequence element to stimulate protein synthesis in cell-free
extracts" Nucleic Acids Res. 35: 411-426
Kim DM, Jun SY, Kang SH
"Fluorescent labeling of cell-free synthesized proteins by incorporation of fluorophore-conjugated nonnatural amino acids" Anal. Biochem. 360: 1-6
2006
Kim DM, Park CG, Choi CY, Oh IS, Keum JW, Kim TW
"Simple procedures for the construction of a
robust and cost-effective cell-free protein synthesis system" J Biotechnol
126: 554-561
Keum JW, Kim DM, Kwon YC, Lee KH, Choi CY, Ahn JH
"The presence of a common downstream box
enables the simultaneous expression of multiple proteins in an E-coli
extract" Biochem Biophys Res Commun. 350: 562-567
Kim DM, Cho CY, Hahn GH, Park KM, Oh IS, Hwang MY, Ahn JH
"Preparation method for Escherichia coliS30
extracts completely dependent upon tRNA addition to catalyze cell-free protein
synthesis"Biotechnol. Bioprocess Eng. 11: 420-424
Choi CY, Park CG, Kim TW, Kim DM
"Providing an oxidizing environment for the
cell-free expression of disulfide-containing proteins by exhausting the
reducing activity of Escherichia coli S30 extract" Biotechnol. Bioprocess
Eng. 22: 1225-1228
Kim DM, Hahn GH
"Production of milligram quantities of
recombinant proteins from PCR-amplified DNAs in a continuous-exchange cell-free
protein synthesis system" Anal. Biochem. 355: 151-153
Kwon YC, Kim DM, Park KM
"PCR 기반의 무세포
단백질 발현 시스템을 이용한 절단 트랜스아미나제의 고속생산" 한국생물공학회지 21(4): 302-305
Kim DM
"Development of a rapid and productive
cell-free protein synthesis system" Bioprocess Eng. 11(3): 235-239
Kim DM
"Rapid production of milligram quantities of
proteins in a batch cell-free protein synthesis system" J Biotechnol
124(2): 373-380
Choi CY, Kim DM, Kim YE
"Zeolite-mediated cation exchange enhances the
stability of mRNA during cell-free protein synthesis" Biotechnol. Bioprocess
Eng. 11: 258-261
Kim DM, Choi CY, Park CG, Hwang MY, Ahn JH, Son JM
"Enhancing the efficiency of cell-free protein
synthesis through the polymerase-chain-reaction-based addition of a translation
enhancer sequence and the in situ removal of the extra amino acid
residues" Anal. Biochem. 351: 187-192
Kim TW, Choi CY, Park CK, Kim DM
"Enhanced Synthesis of Active rPA in the
Continuous Exchange Cell-free Protein Synthesis (CECF) System utilizing
Molecular Chaperones" 한국생물공학회지
21(2): 118-122
Kim DM, Choi CY, Park CK, Kim TW, Hwang MY, Son JM, Ahn JH
"Enhanced in vitro protein synthesis through
optimal design of PCR primers" J. Microbiol. Biotechnol. 16: 355-359
Kim DM, Choi CY, Park CG, Kim TW, Kuem JW
"Oxalate enhances protein synthesis in
cell-free synthesis system utilizing 3-phosphoglycerate as energy source "
J. Biosci. Bioeng. 101: 162-165
Kim DM, Choi CY, Ahn JH, Oh IS, Kim TW
"Cell-free synthesis and in situ isolation of recombinant proteins" Protein Expr. Purif. 45: 249-254
2005
Kim DM, Park CG, Hahn GH, Choi CY, Oh IS, Kim TW, Chu HS, Ahn JH
"Cell-free synthesis of recombinant proteins
from PCR-amplified genes at a comparable productivity to that of plasmid-based
reactions" Biochem Biophys Res Commun. 338: 1346-1352
Kim DM, Choi CY, Ahn JH
"Effect of energy source on the efficiency of
translational termination during cell-free protein synthesis" Biochem
Biophys Res Commun. 337: 325-329
Kim DM, Choi CY, Park CG, Kang TJ, Son JM, Kim TW, Kim NY, Ahn JH
"Effect of temperature and ATP supply on the
efficiency of programmed nonsense suppression" Anal. Biochem. 345: 110-115
Lee KY, Jeon SY, Kim HJ, Kim DM, Kang SH
"Cell-free production of aggregation-prone
proteins in soluble and active forms" BIOTECHNOL PROGR 21(5): 1412-1419
2004
Keum JW, Oh IS, Kim TW, Kim DM, Choi CY
"해당과정의 활성화를 통한 무세포 단백질 발현 시스템에서의 ATP 재생" 한국생물공학회지 19(6): 467-470
Swartz JR, Kim DM
"Efficient production of a bioactive, multiple
disulfide-bonded protein using modified extracts of Escherichia coli"
Biotechnol. Bioeng. 85(2): 122-129
2003
Kim DM
"Cell-free expression of proteins containing
multiple disulfide bonds" Cell-free Protein Expression, Swartz JR ed.
125-131
2002
Kim DM
"Improved composition and energy supply for
bacterial batch systems" Cell-free translation system, Spirin AS ed. 41-52
2001
Kim DM
"Regeneration of ATP for cell-free protein
synthesis from pyruvate and glucose-6-phosphate" Biotechnol. Bioeng. 74:
309-316
2000
Kim DM
"Oxalate improves protein synthesis by
enhancing ATP supply in a cell-free system derived from E.coli" Biotechnol
Lett 22(19): 1537-1542
Kim DM
"Prolonging cell-free protein synthesis by
selective reagent additions" Biotechnol. Prog. 16(3): 385-390
1999
Kim DM
"Prolonging cell-free protein synthesis using
a novel ATP regeneration system" Biotechnol. Bioeng. 66(3): 180-188
1996
Kim DM
"Enhancement of protein synthesis with sodium ion in a cell-free system from Escherichia coli" J. Ferment. Bioeng. 82(4): 398-400
Kim DM
"A semicontinuous prokaryotic coupled transcription/translation system using dialysis membrane"Biotechnol. Prog. 12(5): 645-649
Kim DM
"A highly efficient cell-free protein
synthesis system from Escherichia coli" Eur. J. Biochem. 239: 881-886
1995
Kim DM
"Effect of zeolite on protein synthesis in a cell-free system from E.coli"Biotechnol Lett 17(10): 1043-1046